Check-Direct CPE for BD MAXTM

Easy automated confirmation of CPE with the BD MAXTM

Covers the clinically most prevalent carbapenemases - KPC, OXA-48, VIM and NDM - including the emerging OXA-181 variant.

  • Confirm CPE in routine suspected isolates: detect KPC, OXA-48, VIM and NDM, including OXA-181
  • Identify the carbapenemase type: receive extra information for infection control and epidemiology
  • Pre-aliquoted dried-down reagents ensure a fast and simple testing procedure - just snap in and start

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Check-Direct CPE for BD MAX reduces hands-on processing time, and additionally discriminates VIM and NDM strains

- Williams et al. Poster presented at: BSAC Spring Meeting, 20 March 2014, London, UK.
  • Specimen

    Culture

  • Carbapenemase target genes*

    KPC, OXA-48, VIM, NDM

  • Sample preparation, Amplification and Detection

    BD MAXTM System

  • Controls included

    Positive control
    Sample Processing Control (SPC)

  • Analytical reactivity

    Tested with:
    • 19 different strains of KPC
    • 25 different strains of OXA-48
    • 17 different strains of NDM
    • 34 different strains of VIM

  • Analytical specificity

    No positive results when tested against 132 non-carbapenemase producers

  • Result

    • KPC
    • OXA-48
    • VIM
    • NDM

*KPC-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25; OXA-48, 48b, 162, 163, 181, 204, 232, 244, 245, 370; VIM-1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47; NDM-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16.

Sample preparation

  • Prepare a 0.5-1.0 McFarland bacterial cell suspension using saline
  • Add 10 μl cell suspension and 500 μl PCR-grade water to a Sample Buffer Tube
  • Vortex 10 seconds

BD MAXTM rack setup

  • Position 1:DNA extraction BD Exk-1 Reagent tube (white seal)
  • Position 2: DNA MMK Master Mix tube (green/yellow seal)
  • Position 3: CPE reagent tube (purple seal)

Proceed with the BD MAXTM instrument setup, as specified in the user manual.


Please refer to the full protocol in the user manual when performing this assay.

Confirm CPE in routine suspected isolates

Check-Direct CPE for BD MAXTM is performed on culture specimens.

Detect carbapenemases, including OXA-181

Detect the most clinically prevalent carbapenemases, including emerging variants such as OXA-181. Information on the type of carbapenemase present – KPC, OXA-48-like, VIM and NDM – yields extra information for epidemiology.

Just snap in and start

Pre-aliquoted dried-down reagents ensure a fast and simple testing procedure – just snap in and start.

For countries outside of the United States and the Netherlands (as of 1 August 2016):

Please contact your local BD representative

 BD Catalog Number    Check-Points Catalog Number     Description
 278101  18-0082  Check-Direct CPE for BD MAXTM kit, 24 reactions



For the Netherlands:

Please contact Check-Points directly

 Check-Points Catalog Number   Description
18-0082 Check-Direct CPE for BD MAXTM kit, 24 reactions



Please note that the following reagents and consumables are to be purchased from your local BD representative:

  • BD MAX ExKTM DNA-1 Extraction kit (Ref:442818)
  • BD MAX DNA MMK Master Mix (Ref: 442848)
  • BD MAX PCR Cartridges (Ref: 437519)