Check-Direct CPE Screen for BD MAXTM

Identify CPE carriers with the BD MAXTM directly from swabs

Covers the clinically most prevalent carbapenemases - KPC, OXA-48, VIM and NDM - including the emerging OXA-181 variant.

  • Quick screening directly from rectal swabs helps end unnecessary patient isolation sooner
  • Detect KPC, OXA-48, VIM and NDM, including OXA-181
  • Identify the carbapenemase type: receive extra information for infection control and epidemiology

Downloads

An accurate and rapid method to detect KPC, OXA-48, VIM and NDM genes directly from a rectal screening swab.

- Nijhuis et al. Diagn Microbiol Infect Dis. 2013 Dec;77(4):316-20.
  • Specimen

    Rectal swab

  • Carbapenemase target genes*KPC, OXA-48, VIM, NDM
  • Sample preparation, Amplification and Detection

    BD MAXTM System

  • Controls included

    Positive control
    Sample Processing Control (SPC)

  • Result

    • KPC
    • OXA-48
    • VIM
    • NDM

*KPC-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25; OXA-48, 48b, 162, 163, 181, 204, 232, 244, 245, 370; VIM-1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47; NDM-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16.

Sample preparation

  • Add 25 μl rectal swab specimen contained in transport medium to a Sample Buffer Tube
  • Vortex 10 seconds

BD MAXTM rack setup

  • Position 1:DNA extraction BD Exk-1 Reagent tube (white seal)
  • Position 2: leave empty
  • Position 3: CPE Screen reagent tube (blue seal) +12.5 μl CP Mastermix (included in kit)

Please note: BD MAX DNA MMK Master Mix (Ref: 442848) is not compatible for use with Check-Direct CPE Screen for BD MAXTM.

Proceed with the BD MAXTM instrument setup, as specified in the user manual.


Please refer to the full protocol in the user manual when performing this assay.

Test rectal swabs directly

Identify CPE carriers in specific risk groups. Check-Direct CPE Screen for BD MAXTM is performed directly from rectal swabs.

The most clinically prevalent carbapenemases, including OXA-181

Detect the most clinically prevalent carbapenemases, including emerging variants such as OXA-181. Information on the type of carbapenemase present – KPC, OXA-48-like and VIM and NDM – yields extra information for epidemiology.

End unnecessary patient isolation sooner

Rapid and accurate results help you end unnecessary patient isolation sooner. Stay up-to-date, stay in control.

For countries outside of the United States and the Netherlands (as of 1 August 2016):

Please contact your local BD representative

 BD Catalog Number  
Check-Points Catalog Number   Description 
 278100  18-0051  Check-Direct CPE Screen for BD MAXTM kit, 24 reactions



For the Netherlands:

Please contact Check-Points directly

 Check-Points Catalog Number     Description
18-0051 Check-Direct CPE Screen for BD MAXTM kit, 24 reactions



Please note: BD MAX DNA MMK Master Mix (Ref: 442848) is not compatible for use with Check-Direct CPE Screen for BD MAXTM. Instead please use the "CP Mastermix" included in the Check-Direct CPE Screen for BD MAXTM kit.

The following additional reagents and consumables are to be purchased from your local BD representative:

  • BD MAX ExKTM DNA-1 Extraction kit (Ref:442818)
  • BD MAX PCR Cartridges (Ref: 437519)