Check-MDR CT101

Investigate the epidemiology of suspected ESBL- and AmpC-producers with a single test

Stay on top of the epidemiology in your healthcare facility and make sure to keep antibiotic resistance at a low level. Prevention starts early.

  • Discriminate directly between ESBL and non-ESBL variants of TEM and SHV
  • Identify presumptive mobile AmpCs
  • Obtain objective results and improve traceability with the E-Ads software

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The fact that multiple resistance genes were correctly identified whatever the complex genetic background of the tested isolates…confirms that the Check-Points array technology is a highly accurate tool for detection of coexistent bla genes

- Bogaerts et al. Antimicrob Agents Chemother. 2011 Sep;55(9):4457-60.
  • Carbapenemases*

    KPC, NDM

  • CTX-M ESBLs

    CTX-M-1 group, CTX-M-2 group, CTX-M-8 & -25 group, CTX-M-9 group

  • TEM ESBLs vs. non-ESBL

    TEM wt, TEM E104K, TEM R164S, TEM R164H, TEM G238S

  • SHV ESBL vs. non-ESBL

    SHV wt, SHV G238S, SHV G238A, SHV E240K

  • AmpCs

    CMY I/MOX, ACC, DHA, ACT/MIR, CMY II, FOX

  • Controls included

    DNA control, Amplification control, Hydridization control, Negative control

  • Specimen

    Culture

  • Sample preparation

    Magnetic bead- or column-based methods**

  • Pre-PCR equipment

    Thermocycler**, vortex mixer, mini-centrifuge

  • Post-PCR equipment

    Thermocycler**, vortex mixer, mini-centrifuge, thermomixer with active cooling**, Check-Points Tube Reader including E-Ads software, computer with USB drive and internet connection, barcode reader (optional)

  • Throughput

    3 to 72 samples/run

*KPC-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25; NDM-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16.
**contact your local representative for specifications

  Time per step  Hands-on time per step 
1. Culture    

2. DNA extraction
of total nucleic acid using automated, magnetic bead or column-based methods (not supplied)

   

3. Identification
by multiplex ligation

3 h 15 min

4. Amplification
of ligated probes by PCR

1.5 h

15 min

5. Detection
through hybridization of amplified probes to specific locations on the microarray, contained in a Check-Points Array Tube
2 h 30 min
 6. Results
are generated using the Check-Points Tube Reader to produce an image of the microarray and the E-Ads software to automatically translate this image into the presence or absence of specific beta-lactamase genes
   

TEM and SHV ESBL vs. non-ESBL

Discriminate directly between ESBL and non-ESBL variants of TEM and SHV, eliminating the need for additional confirmatory sequencing. By employing a highly specific ligation reaction, wild type TEM and SHV and the exact point mutations that result in an ESBL phenotype can be accurately detected and differentiated.

Mobile AmpCs

Identify presumptive mobile AmpCs, generally associated with significantly higher beta-lactamase production than their chromosomally encoded counterparts. The finding of a particular AmpC variant in a bacterial species that naturally does not harbor it, indicates the presence of a mobile AmpC.

Objective results

Obtain objective results and improve traceability with the E-Ads software. Data is displayed immediately onscreen, summarized in a convenient format after each run and may be accessed anytime via the E-Ads software database.

Downloads


Technology

  • Learn more about the technology behind our Check-MDR CT microarray assays here


Posters


Publications

  • Castanheira M, Mills JC, Costello SE, Jones RN, Sader HS. Ceftazidime-Avibactam Activity Tested against Enterobacteriaceae Isolates from U.S. Hospitals (2011 to 2013) and Characterization of β-Lactamase-Producing Strains. Antimicrob Agents Chemother. 2015 Jun;59(6):3509-17.
    Learn more
  • Bogaerts P, Hujer AM, Naas T, de Castro RR, Endimiani A, Nordmann P, Glupczynski Y, Bonomo RA. Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from beta-lactam-resistant gram-negative bacteria. Antimicrob Agents Chemother. 2011 Sep;55(9):4457-60.
    Learn more
  • Castanheira M, Farrell SE, Deshpande LM, Mendes RE, Jones RN. Prevalence of β-lactamase encoding genes among Enterobacteriaceae bacteremia isolates collected in 26 USA hospitals: Report from the SENTRY Antimicrobial Surveillance Program (2010). Antimicrob Agents Chemother. 2013 Jul;57(7):3012-20.
    Learn more
  •  Lascols C, Hackel M, Marshall SH, Hujer AM, Bouchillon S, Badal R, Haban D, Bonomo RA. Increasing prevalence and dissemination of NDM-1 metallo-β-lactamase in India: data from the SMART study (2009). J Antimicrob Chemother. 2011 Sep;66(9):1992-7.
    Learn more
  • De Boeck H, Lunguya O, Muyembe JJ, Glupczynski Y, Jacobs J. Presence of extended-spectrum beta-lactamase-producing Enterobacteriaceae in waste waters, Kinshasa, the Democratic Republic of the Congo. Eur J Clin Microbiol Infect Dis. 2012 Nov;31(11):3085-8.
    Learn more
Catalog number Description
10-0020  Check-MDR CT101 kit, 72 reactions
 Accessories:  
 16-0012  Check-Points Tube Reader incl. E-Ads Software

Contact your local representative

For information about further equipment and supplies needed to run the assay, please refer to the “Materials required but not supplied” section of the user manual.