Check-MDR CT102

Your tool for characterizing carbapenem-resistant Enterobacteriaceae (CRE)

Find out which carbapenemase and/or ESBL types are present. Clear answers are delivered within a few hours.

  • Detect the clinically most prevalent carbapenemases and ESBLs in Enterobacteriaceae
  • Discriminate directly between ESBL and non-ESBL variants of TEM and SHV
  • Obtain objective results and improve traceability with the E-Ads software

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Its use in clinical practice may contribute to better knowledge of local epidemiology of β-lactam resistance genes and to more appropriate use of antimicrobial agents, reduction of costs, and improved patient outcome.

- Naas et al. J Clin Microbiol. 2011 Apr;49(4):1608-13.
  • Carbapenemases*

    KPC, NDM, VIM, IMP, OXA-48-like

  • CTX-M ESBLs

    CTX-M-1 group, CTX-M-2 group, CTX-M-8 & -25 group, CTX-M-9 group

  • TEM ESBLs vs. non-ESBL

    TEM wt, TEM E104K, TEM R164S, TEM R164H, TEM G238S

  • SHV ESBL vs. non-ESBL

    SHV wt, SHV G238S, SHV G238A, SHV E240K

  • Controls included

    DNA control, Amplification control, Hybridization control, Negative control

  • Specimen

    Culture

  • Sample preparation

    Magnetic bead- or column-based methods**

  • Pre-PCR equipment

    Thermocycler**, vortex mixer, mini-centrifuge

  • Post-PCR equipment

    Thermocycler**, vortex mixer, mini-centrifuge, thermomixer with active cooling**, Check-Points Tube Reader including E-Ads software, computer with USB drive and internet connection, barcode reader (optional)

  • Throughput

    3 to 72 samples/run

*KPC-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25; OXA-48, 48b, 162, 163, 181, 204, 232, 244, 245, 370; VIM-1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47; NDM-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16; IMP- 1, 3, 6, 7, 8, 10, 13, 19, 20, 24, 30, 37, 40, 42.
**contact your local representative for specifications

  Time per step  Hands-on time per step 
1. Culture    

2. DNA extraction
of total nucleic acid using automated, magnetic bead or column-based methods (not supplied)

   

3. Identification
by multiplex ligation

3 h 15 min

4. Amplification
of ligated probes by PCR

1.5 h

15 min

5. Detection
through hybridization of amplified probes to specific locations on the microarray, contained in a Check-Points Array Tube
2 h 30 min
 6. Results
are generated using the Check-Points Tube Reader to produce an image of the microarray and the E-Ads software to automatically translate this image into the presence or absence of specific beta-lactamase genes
   

Carbapenemases and ESBLs

Detect the clinically most prevalent carbapenemases and ESBLs in Enterobacteriaceae. The microarray platform allows a broad range of relevant targets to be analyzed simultaneously and is also well-suited to detect co-resistance.

TEM and SHV ESBL vs. non-ESBL

Discriminate directly between ESBL and non-ESBL variants of TEM and SHV, eliminating the need for additional confirmatory sequencing. By employing a highly specific ligation reaction, wild type TEM and SHV and the exact point mutations that result in an ESBL phenotype can be accurately detected and differentiated.

Objective results

Obtain objective results and improve traceability with the E-Ads software. Data is displayed immediately onscreen, summarized in a convenient format after each run and may be accessed anytime via the E-Ads software database.

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Technology

  • Learn more about the technology behind our Check-MDR CT microarray assays here


Posters


Publications

  • Woodford N, Warner M, Pike R, Zhang J. Evaluation of a commercial microarray to detect carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother. 2011 Dec;66(12):2887-8.
    Learn more
  • Naas T, Cuzon G, Bogaerts P, Glupczynski Y, Nordmann P. Evaluation of a DNA microarray (Check-MDR CT102) for rapid detection of TEM, SHV, and CTX-M extended-spectrum β-lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapenemases. J Clin Microbiol. 2011 Apr;49(4):1608-13.
    Learn more
  • Cohen Stuart J, Voets G, Scharringa J, Fluit A, Leverstein-Van Hall M. Detection of carbapenemase producing Enterobacteriaceae with a commercial DNA Microarray. J Med Microbiol. 2012 Jun;61(Pt 6):809-12.
    Learn more
  • Somily AM, Arshad MZ, Garaween GA, Senok AC. Phenotypic and genotypic characterization of extended-spectrum b-lactamases producing Escherichia coli and Klebsiella pneumoniaein a tertiary care hospital in Riyadh, Saudi Arabia. Ann Saudi Med. 2015 Nov-Dec;35(6):435-9.
    Learn more
  • Somily AM, Garaween GA, Abukhalid N, Absar MM, Senok AC. Comparison of Molecular and Phenotypic Methods for the Detection and Characterization of Carbapenem Resistant Enterobacteriaceae. Acta Microbiol Immunol Hung. 2016 Mar;63(1):69-81.
    Learn more


Catalog number Description
10-0019  Check-MDR CT102 kit, 72 reactions
 Accessories:  
 16-0012  Check-Points Tube Reader incl. E-Ads Software

Contact your local representative

For information about further equipment and supplies needed to run the assay, please refer to the “Materials required but not supplied” section of the user manual.