Your tool for characterizing carbapenem-resistant Enterobacteriaceae (CRE)
Find out which carbapenemase and/or ESBL types are present. Clear answers are delivered within a few hours.
- Detect the clinically most prevalent carbapenemases and ESBLs in Enterobacteriaceae
- Discriminate directly between ESBL and non-ESBL variants of TEM and SHV
- Obtain objective results and improve traceability with the E-Ads software
Its use in clinical practice may contribute to better knowledge of local epidemiology of β-lactam resistance genes and to more appropriate use of antimicrobial agents, reduction of costs, and improved patient outcome.
KPC, NDM, VIM, IMP, OXA-48-like
- CTX-M ESBLs
CTX-M-1 group, CTX-M-2 group, CTX-M-8 & -25 group, CTX-M-9 group
- TEM ESBLs vs. non-ESBL
TEM wt, TEM E104K, TEM R164S, TEM R164H, TEM G238S
- SHV ESBL vs. non-ESBL
SHV wt, SHV G238S, SHV G238A, SHV E240K
- Controls included
DNA control, Amplification control, Hybridization control, Negative control
- Sample preparation
Magnetic bead- or column-based methods**
- Pre-PCR equipment
Thermocycler**, vortex mixer, mini-centrifuge
- Post-PCR equipment
Thermocycler**, vortex mixer, mini-centrifuge, thermomixer with active cooling**, Check-Points Tube Reader including E-Ads software, computer with USB drive and internet connection, barcode reader (optional)
3 to 72 samples/run
*KPC-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25; OXA-48, 48b, 162, 163, 181, 204, 232, 244, 245, 370; VIM-1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47; NDM-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16; IMP- 1, 3, 6, 7, 8, 10, 13, 19, 20, 24, 30, 37, 40, 42.
**contact your local representative for specifications
|Time per step||Hands-on time per step|
2. DNA extraction
|3 h||15 min|
through hybridization of amplified probes to specific locations on the microarray, contained in a Check-Points Array Tube
|2 h||30 min|
| 6. Results
are generated using the Check-Points Tube Reader to produce an image of the microarray and the E-Ads software to automatically translate this image into the presence or absence of specific beta-lactamase genes
Carbapenemases and ESBLs
Detect the clinically most prevalent carbapenemases and ESBLs in Enterobacteriaceae. The microarray platform allows a broad range of relevant targets to be analyzed simultaneously and is also well-suited to detect co-resistance.
TEM and SHV ESBL vs. non-ESBL
Discriminate directly between ESBL and non-ESBL variants of TEM and SHV, eliminating the need for additional confirmatory sequencing. By employing a highly specific ligation reaction, wild type TEM and SHV and the exact point mutations that result in an ESBL phenotype can be accurately detected and differentiated.
Obtain objective results and improve traceability with the E-Ads software. Data is displayed immediately onscreen, summarized in a convenient format after each run and may be accessed anytime via the E-Ads software database.
- Learn more about the technology behind our Check-MDR CT microarray assays here
- Eigner U, Bertsch D, Moskorz K, Holfelder M. Evaluation of a low-density microarray for the identification of ESBL, AmpC and carbapeneamase-producing Enterobacteriaceae. Presented at: 21st ECCMID; 2011 May 7-10; Milan, Italy.
- Woodford N, Warner M, Pike R, Zhang J. Evaluation of a commercial microarray to detect carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother. 2011 Dec;66(12):2887-8.
- Naas T, Cuzon G, Bogaerts P, Glupczynski Y, Nordmann P. Evaluation of a DNA microarray (Check-MDR CT102) for rapid detection of TEM, SHV, and CTX-M extended-spectrum β-lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapenemases. J Clin Microbiol. 2011 Apr;49(4):1608-13.
- Cohen Stuart J, Voets G, Scharringa J, Fluit A, Leverstein-Van Hall M. Detection of carbapenemase producing Enterobacteriaceae with a commercial DNA Microarray. J Med Microbiol. 2012 Jun;61(Pt 6):809-12.
- Somily AM, Arshad MZ, Garaween GA, Senok AC. Phenotypic and genotypic characterization of extended-spectrum b-lactamases producing Escherichia coli and Klebsiella pneumoniaein a tertiary care hospital in Riyadh, Saudi Arabia. Ann Saudi Med. 2015 Nov-Dec;35(6):435-9.
- Somily AM, Garaween GA, Abukhalid N, Absar MM, Senok AC. Comparison of Molecular and Phenotypic Methods for the Detection and Characterization of Carbapenem Resistant Enterobacteriaceae. Acta Microbiol Immunol Hung. 2016 Mar;63(1):69-81.
|10-0019||Check-MDR CT102 kit, 72 reactions|
|16-0012||Check-Points Tube Reader incl. E-Ads Software|
For information about further equipment and supplies needed to run the assay, please refer to the “Materials required but not supplied” section of the user manual.
Other Check-Points solutions
EU: For in vitro Diagnostic Use.
US: For Research Use Only. Not for use in diagnostic procedures.