Check-MDR CT103 XL

The most comprehensive CE-IVD assay for molecular beta-lactamase identification

Includes new carbapenemase and ESBL targets, including emerging types and those typically found in non-fermenters. Stay up-to-date, stay in control.

  • Now extended with carbapenemases typically identified in Acinetobacter baumannii such as OXA-23, OXA-24/40 and OXA-58 as well as carbapenemases and ESBLs found in Pseudomonas aeruginosa
  • Includes emerging carbapenemases (GIM, GES, SPM) and ESBLs (VEB, PER, BEL, GES)
  • Discriminate directly between carbapenemase and ESBL variants of GES

Downloads

The Check-MDR CT103 accurately typed 106/109 (97%) isolates.…Notably, 42 isolates were found to contain resistance genes not originally known to be present, each of which was confirmed by subsequent conventional PCR.

- Cunningham et al. Presented at: 54th ICAAC; 2014 Sept 5-9; Washington, US.
  • Carbapenemases*

    KPC, NDM, VIM, IMP, OXA-48-like, GES, GIM, SPM, OXA-23-like, OXA-24/40-like, OXA-58-like

  • CTX-M ESBLs

    CTX-M-1 group, CTX-M-1-like, CTX-M-15-like, CTX-M-3-like, CTX-M-32-like, CTX-M-2 group, CTX-M-8 & -25 group, CTX-M-9 group

  • TEM ESBLs vs. non-ESBL

    TEM wt, TEM E104K, TEM R164S, TEM R164C, TEM R164H, TEM G238S

  • SHV ESBLs vs. non-ESBL

    SHV wt, SHV G238S, SHV G238A, SHV E240K

  • Other ESBLs**

    VEB, PER, BEL, GES

  • AmpCs

    CMY I/MOX, ACC, DHA, ACT/MIR, CMY II, FOX

  • Controls included

    DNA control, Amplification control, Hydridization control, Negative control

  • Specimen

    Culture

  • Sample preparation

    Magnetic bead- or column-based methods***

  • Pre-PCR equipment

    Thermocycler***, vortex mixer, mini-centrifuge

  • Post-PCR equipment

    Thermocycler***, vortex mixer, mini-centrifuge, thermomixer with active cooling***, Check-Points Tube Reader including E-Ads software, computer with USB drive and internet connection, barcode reader (optional)

  • Throughput

    1 to 24 samples/run

*KPC-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25; OXA-48, 48b, 162, 163, 181, 204, 232, 244, 245, 370; VIM-1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47; NDM-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16; IMP- 1, 3, 6, 7, 8, 10, 13, 19, 20, 24, 30, 37, 40, 42; GES-2, 4, 5, 6, 13, 14, 15, 16, 18, 20, 21; GIM-1, SPM-1; OXA-23, 27, 49, 73, 146, 165, 166, 167, 168, 169, 170, 171, 225, 239; OXA-24, 25, 26, 33, 40, 72, 139, 207; OXA-58, 96, 164.

**VEB-1, 2, 3, 4, 5, 6, 7, 8; PER-1, 2, 3, 4, 5, 6; BEL-1, 2, 3; GES-1, 3, 7, 8, 9, 10, 11, 12, 17, 19, 22.

***contact your local representative for specifications

 

Time per step 

Hands-on time per step 

1. Culture

 

 

2. DNA extraction
of total nucleic acid using automated, magnetic bead or column-based methods (not supplied)

 

 

3. Identification
by multiplex ligation

3 h

15 min

4. Amplification
of ligated probes by PCR

1.5 h

15 min

5. Detection
through hybridization of amplified probes to specific locations on the microarray, contained in a Check-Points Array Tube

2 h

30 min

 6. Results
are generated using the Check-Points Tube Reader to produce an image of the microarray and the E-Ads software to automatically translate this image into the presence or absence of specific beta-lactamase genes

 

 

Enterobacteriaceae & non-fermenters

Detect beta-lactamases commonly encountered in non-fermenters and Enterobacteriaceae. Now extended with carbapenemases typically identified in Acinetobacter baumannii such as OXA-23, OXA-24/40 and OXA-58 as well as carbapenemases and ESBLs found in Pseudomonas aeruginosa.

Emerging or less common carbapenemases & ESBLs

Keep a lookout for less common or emerging carbapenemases and ESBLs. These new targets, selected in close collaboration with leading experts,  help you stay in control and include GIM, GES and SPM carbapenemases and VEB, PER, BEL and GES ESBLs.

GES carbapenemases vs. ESBLs

Discriminate directly between carbapenemase and ESBL variants of GES, eliminating the need for additional confirmatory sequencing. By employing a highly specific ligation reaction, the point mutations in GES ESBL that give rise to a carbapenemase phenotype can be accurately detected..

CTX-M-1 family subgrouping

Receive guidance for further characterization of the CTX-M-1 group enzymes. This globally dominating CTX-M group can now be subdivided into four groups.

TEM and SHV ESBL vs. non-ESBL

Discriminate directly between ESBL and non-ESBL variants of TEM and SHV, eliminating the need for additional confirmatory sequencing. By employing a highly specific ligation reaction, wild type TEM and SHV and the exact point mutations that result in an ESBL phenotype can be accurately detected and differentiated.

Mobile AmpCs

Identify presumptive mobile AmpCs, generally associated with significantly higher beta-lactamase production than their chromosomally encoded counterparts. The finding of a particular AmpC variant in a bacterial species that naturally does not harbor it, indicates the presence of a mobile AmpC.

Objective results

Obtain objective results and improve traceability with the E-Ads software. Data is displayed immediately onscreen, summarized in a convenient format after each run and may be accessed anytime via the E-Ads software database.

Discover more with a comprehensive test

The microarray format allows you to investigate a large range of targets simultaneously and get a more complete picture of the epidemiological situation. Discover more1 with a comprehensive test.

 

 1. Cunningham SA, Johnston B, Vasoo S, Johnson J, Patel R. Evalutaion of Check-Points Check-MDR CT103 PCR-Microarray Kit for Detection and Classification of ESBL, AmpC and Carbapenemase Genes. Presented at: 54th ICAAC; 2014 Sept 5 -9; Washington, DC.

Catalog number Description
10-0022  Check-MDR CT103 XL kit, 24 reactions
 Accessories:  
 16-0012  Check-Points Tube Reader incl. E-Ads Software

Contact your local representative

For information about further equipment and supplies needed to run the assay, please refer to the “Materials required but not supplied” section of the user manual.